Status dan Mekanisme Resistensi Biokimia Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) terhadap Insektisida Organofosfat serta Kepekaannya terhadap Insektisida Botani Ekstrak Biji Barringtonia asiatica

Authors

  • DANAR DONO Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian Universitas Padjadjaran
  • SYAFRI ISMAYANA Jurusan Kimia Fakultas MIPA Universitas Padjadjaran
  • IDAR IDAR Jurusan Kimia Fakultas MIPA Universitas Padjadjaran
  • DJOKO PRIJONO Departemen Proteksi Tanaman Fakultas Pertanian Institut Pertanian Bogor
  • IKHA MUSLIKHA Alumnus Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian Universitas Padjadjaran

DOI:

https://doi.org/10.5994/jei.7.1.9

Keywords:

resistance, enzyme, organophosphat, botanical insecticide

Abstract

An examination of insect resistance was determined by several steps, i.e. standard sensitivity, resistance diagnosis, and determination of resistance level. Each phase was tested with feeding and residue contact methods at glass tube. Resistance ratio (RR) was determined by comparing LC50 value of field population with standard population. Field population of C. pavonana was classified resistant if it had RR 5 4. Biochemistry analysis of resistance was conducted to population of C. pavonana showing resistance to prophenophos insecticide. The activity analysis of acetylcholine esterase (ACHE), esterase, and Glutation Stransferase was done with spectrophotometer method. Insect which are resistant to prophenophos insecticide was tested for its sensitivity to Barringtonia asiatica seed extract. Result indicated that C. pavonana population from Pengalengan showed resistance to prophenophos synthetic insecticide. Using contact test, the highest resistance ratio value was 4.04, while by feeding assay the RR was 2.78. The study on biochemical resistance mechanisms of each field population of C. pavonana showed various activities of enzymatic detoxification. This could be due to the difference in the kind of insecticides exposed to each field population of C. pavonana. Since RR value from the contact test was higher than that of the feeding test, the resistance development of C. pavonana to synthetic insecticides was probably caused by physiological and biochemical changes in insect cuticle rather than the activity of detoxification enzyme. Methanolic seed extract of B. asiatica can be used as an alternative of resistance management of C. pavonana to prophenophos synthetic insecticide.
JEI Volume 7 No. 1, April 2010

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Published

2015-09-28

How to Cite

DONO, D., ISMAYANA, S., IDAR, I., PRIJONO, D., & MUSLIKHA, I. (2015). Status dan Mekanisme Resistensi Biokimia Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) terhadap Insektisida Organofosfat serta Kepekaannya terhadap Insektisida Botani Ekstrak Biji Barringtonia asiatica. Jurnal Entomologi Indonesia, 7(1), 9. https://doi.org/10.5994/jei.7.1.9

Issue

Vol. 7 No. 1 (2010): April

Section

Articles

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