The effectiveness of clove oil, its fractions, and clove oil-based fumigant tablet formulations, against Tribolium castaneum (Herbst)

Authors

  • Trijanti Anughrahiyaharti Widinni Asnan Laboratorium Entomologi, Southeast Asian Regional Centre for Tropical Biology (SEAMEO BIOTROP), Indonesia
  • Sri Widayanti Laboratorium Entomologi, Southeast Asian Regional Centre for Tropical Biology (SEAMEO BIOTROP), Indonesia
  • Idham Sakti Harahap Laboratorium Entomologi, Southeast Asian Regional Centre for Tropical Biology (SEAMEO BIOTROP), Indonesia; Departemen Proteksi Tanaman, Fakultas Perhatian, IPB University, Indonesia
  • Herni Widhisatuti Laboratorium Entomologi, Southeast Asian Regional Centre for Tropical Biology (SEAMEO BIOTROP), Indonesia

DOI:

https://doi.org/10.5994/jei.18.3.191

Keywords:

fractionation, mortality, naphthalene, stored product insect

Abstract

Indonesia has seen an increase and widespread reports of resistance among stored-product insect pests to phosphine. The use of Syzigium aromaticum (clove oil) as an alternative fumigant may be a useful strategy to control infestation by phosphine-resistant varieties of stored-product insects. The objective of this study was to examine the effectiveness of whole (unfractionated) clove oil as well as its component fractions as a fumigant and repellent against the red flour beetle (Tribolium castaneum (Herbst)), and to develop a simple fumigant formulation for this purpose. The experimental design used to test the effectiveness of clove oil and its fractionation was a completely randomized design (CRD). Meanwhile, testing the effectiveness of tablet formulations was carried out by factorial CRD. Fumigation test results gave LD50 and LD95 values of 0.234 and 1.142 ml/l respectively, for crude clove oil used in a fumigation chamber against T. castaneum. An n-hexane fraction of clove oil tested under the same conditions was more lethal, causing 95% mortality of T. castaneum at the dose of 0.801 ml/l during fumigation. Finally, tablets containing a set proportion of clove oil and naphthalene (1:1) reached LD91 against T. castaneum after 7 days’ fumigation.

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Published

2021-12-23

How to Cite

Asnan, T. A. W., Widayanti, S. ., Harahap, I. S. ., & Widhisatuti, H. . (2021). The effectiveness of clove oil, its fractions, and clove oil-based fumigant tablet formulations, against Tribolium castaneum (Herbst). Jurnal Entomologi Indonesia, 18(3), 191. https://doi.org/10.5994/jei.18.3.191

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