Jurnal Entomologi Indonesia https://jurnal.pei-pusat.org/index.php/jei <p><strong>Jurnal Entomologi Indonesia </strong>(<a href="https://portal.issn.org/resource/ISSN/1829-7722#">P-ISSN: 1829-7722</a>; <a href="https://portal.issn.org/resource/ISSN/2089-0257">E-ISSN: 2089-0257</a>) is an Indonesian-based scientific journal, published by the <a href="http://pei-pusat.org/">Entomological Society of Indonesia</a>. JEI is issued every March, July, and November of each year. It publishes original research article, reviews, and short communications covering research results in the field of tropical entomology. The JEI was founded in 2004 and is now in its 18th year. The journal is supported by the <a href="http://pei-pusat.org/">Entomological Society of Indonesia</a> in collaboration with the <a href="https://ptn.ipb.ac.id/cms/en/home">Department of Plant Protection</a>, Faculty of Agriculture, IPB University.</p> <p>JEI is an open-access, peer-reviewed journal that has been Accredited (2022-2026) base on the Ministerial Decree of the Minister of Research and Technology/Head of National Research and Innovation Agency of the Republic of Indonesia No. 105/E/KPT/2022</p> <p>The JEI has been registered in Crossref, DOAJ, <a href="https://sinta.kemdikbud.go.id/journals/profile/1058" target="_blank" rel="noopener">SINTA Rank 2</a>, Google Scholar, Portal Garuda, CiteFactor, and other scientific databases. </p> en-US <p>Authors who publish with this journal agree to the following terms:</p> <ol> <li>Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a <a href="http://creativecommons.org/licenses/by/4.0/" rel="license">Creative Commons Attribution 4.0 International License</a> that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.</li> <li>Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.</li> <li>Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See <a href="http://opcit.eprints.org/oacitation-biblio.html" target="_new">The Effect of Open Access</a>).</li> </ol> editor@jurnal.pei-pusat.org (Prof. Dr. Damayanti Buchori, MSc.) editor.jei@gmail.com (Editorial Office) Tue, 30 Apr 2024 16:43:09 +0000 OJS 3.2.1.3 http://blogs.law.harvard.edu/tech/rss 60 The characterization of Apis cerana and Apis mellifera mrjp2 gene in Indonesia and the phylogeny relationship with mrjp family genes https://jurnal.pei-pusat.org/index.php/jei/article/view/812 <p>The <em>major royal jelly protein 2</em> (<em>mrjp2</em>) gene is one of the molecular markers that can discriminate between <em>Apis cerana</em> Fabricius and <em>A. mellifera </em>Linnaeus. Due to the lack of <em>mrjp</em>2 gene sequences registered in GenBank for Indonesian <em>A. cerana</em> and <em>A. mellifera</em>, DNA characterization and bioinformatics analysis were needed. This research aimed to characterize the exon-intron organization of <em>mrjp2</em> genes for both Indonesian bee species and analyze the phylogenetic relationship with other <em>mrjp</em> family genes. DNA samples of <em>Apis cerana</em> and <em>A. mellifera,</em> collected from Bogor, were amplified using MF-MR primer at annealing temperatures of 47 <sup>o</sup>C and 50 <sup>o</sup>C, respectively. The length of <em>A. cerana</em> and <em>A. mellifera</em> DNA sequences were 579 and 597 bp, respectively. The DNA sequences of both species were comprised of partial exons 1, introns 1, exons 2, introns 2, and partial exon 3. The number of putative amino acids of <em>A. cerana</em> and <em>A. mellifera</em> <em>mrjp</em>2 genes were 111 and 123, respectively. We confirmed that the partial MRJP2 putative amino acids of both honey bees belong to the MRJP family and contained the peptide signal in the 14 first amino acid sites. Nucleotide variation of <em>the mrjp2</em> gene in <em>A. cerana</em> was higher than <em>A. mellifera</em>. Phylogenetic tree construction showed that <em>A. cerana</em> and <em>A. mellifera </em>form a monophyletic clade with the <em>A. mellifera</em> <em>mrjp7</em> gene and another <em>mrjp</em> family gene clustered as reported in a previous study.</p> Nurul Insani Shullia, Tia Vina Febiriani, Rika Raffiudin, Candra Widjaja, Tri Atmowidi Copyright (c) 2024 Nurul Insani Shullia, Tia Vina Febiriani, Rika Raffiudin, Candra Widjaja, Tri Atmowidi https://creativecommons.org/licenses/by/4.0 https://jurnal.pei-pusat.org/index.php/jei/article/view/812 Tue, 30 Apr 2024 00:00:00 +0000