Jurnal Entomologi Indonesia https://jurnal.pei-pusat.org/index.php/jei <p><strong>Jurnal Entomologi Indonesia </strong>(<a href="https://portal.issn.org/resource/ISSN/1829-7722#">P-ISSN: 1829-7722</a>; <a href="https://portal.issn.org/resource/ISSN/2089-0257">E-ISSN: 2089-0257</a>) is an Indonesian-based scientific journal, published by the <a href="http://pei-pusat.org/">Entomological Society of Indonesia</a>. JEI is issued every March, July, and November of each year. It publishes original research article, reviews, and short communications covering research results in the field of tropical entomology. JEI was founded in 2004 and is now in its 18th year. The journal is supported by the <a href="http://pei-pusat.org/">Entomological Society of Indonesia</a> in collaboration with the <a href="https://ptn.ipb.ac.id/cms/en/home">Department of Plant Protection</a>, Faculty of Agriculture, IPB University.</p> <p>JEI is an open-access, peer-reviewed journal that has been Accredited (2022-2026) base on Ministerial Decree of the Minister of Research and Technology/Head of National Research and Innovation Agency of the Republic of Indonesia No. 105/E/KPT/2022</p> <p>JEI has been registered in Crossref, DOAJ, <a href="https://sinta.kemdikbud.go.id/journals/profile/1058" target="_blank" rel="noopener">SINTA Rank 2</a>, Google Scholar, Portal Garuda, CiteFactor, and other scientific databases. </p> Perhimpunan Entomologi Indonesia en-US Jurnal Entomologi Indonesia 1829-7722 <p>Authors who publish with this journal agree to the following terms:</p> <ol> <li>Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a <a href="http://creativecommons.org/licenses/by/4.0/" rel="license">Creative Commons Attribution 4.0 International License</a> that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.</li> <li>Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.</li> <li>Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See <a href="http://opcit.eprints.org/oacitation-biblio.html" target="_new">The Effect of Open Access</a>).</li> </ol> The polymorphism of vespid wasps (Hymenoptera: Vespidae) from Indonesia with morphology-based cladistic https://jurnal.pei-pusat.org/index.php/jei/article/view/775 <p>Wasps in the family of Vespidae often have a yellowish black color with white or brown markings, but some species have variations in their markings and coloring. There is limited information available about the distribution of these marking patterns within the Vespidae Family. To clarify their taxonomic status, this study aims to examine the marking patterns of Vespid wasps that are widely distributed in the Indonesian Archipelago and to explore correlations with their biogeographical distribution patterns. In this study, specimens from three different locations (Bandung, Sumedang, and Purworejo) and specimens from the Museum Zoologicum Bogoriense (MZB) were examined. A total of 31 individuals from five different species were analyzed based on their morphological features. The data collected was coded and analyzed using cladistic methods, and the results were used to create cladogram trees for each of the five species i.e., <em>Phimenes flavopictus</em>,<em> Polistes stigma</em>,<em> Apodynerus troglodytes</em>,<em> Vespa affinis</em>, and<em> Vespa velutina</em>, showing their biogeographical distribution. The cladistic analysis in this study showed the relationships between the different species based on their morphological characteristics. The arrangements of the clades were determined using the Euclidean method in R studio. The results showed that <em>P. flavopictus</em> has two clades based on the marking patterns on their thorax and abdomen, while the other species i.e., <em>Po. stigma</em>,<em> V. affinis</em>,<em> V. velutina</em>, and <em>A. troglodytes</em> have three clades based on their coloring and marking patterns on all segments. The distribution of the Vespidae species appears to be scattered, with their patterns randomly distributed among locations.</p> Yelsha Ramadhila Hari Nugroho Erfanus Jane Intan Ahmad Copyright (c) 2023 Yelsha Ramadhila, Hari Nugroho, Erfanus Jane, Intan Ahmad https://creativecommons.org/licenses/by/4.0 2023-08-14 2023-08-14 20 2 101 101 10.5994/jei.20.2.101 Kajian aspek keamanan nyamuk Aedes aegypti Linnaeus ber-Wolbachia di Yogyakarta, Indonesia https://jurnal.pei-pusat.org/index.php/jei/article/view/784 <p>Dengue prevention efforts are limited to the control strategies of its vector and the management of breeding sites. New alternatives for dengue vector control that are sustainable and more environmentally friendly are needed to complement the government’s current efforts. Research on <em>Wolbachia</em>-infected <em>Aedes aegypti</em> Linnaeus mosquitoes as an alternative biocontrol strategy has been performed in Yogyakarta City. However, one of the concerns of the community members and stakeholders about this technology is the safety aspect regarding the transmission of <em>Wolbachia</em> to other species and the possibility that humans will contract <em>Wolbachia</em>. This study aimed to address these concerns, namely to find out whether horizontal transmission of <em>Wolbachia</em> occurred from <em>A. aegypti</em> that were released to other species and whether residents living in the released areas were infected with <em>Wolbachia</em>. The research was conducted in Dusun Nogotirto and Dusun Kronggahan (Sleman Regency), as well as in Dusun Jomblangan and Dusun Singosaren (Bantul Regency), Yogyakarta Special Province. <em>Wolbachia</em> qPCR screening using the target gene WD0513 was performed on 922 <em>Culex quinquefasciatus</em> Say and 331 <em>Aedes albopictus </em>(Skuse). ELISA test was carried out on 190 pairs of plasma samples, namely the sample before the <em>Wolbachia</em> frequency was established (still &lt;80%) and the sample after it was established (<u>&gt;</u>80%). The results showed no evidence of <em>Wolbachia</em> transfer from <em>Wolbachia</em>-infected <em>A. aegypti</em> to other mosquito species coexisting in the same habitat or to humans. This study corroborates the safety evidence of <em>Wolbachia</em>-infected <em>A. aegypti</em> technology as an alternative to control dengue virus transmission</p> Utari Saraswati Endah Supriyati Ayu Rahayu Anwar Rovik Irianti Kurniasari Rio Hermantara Dian Aruni Kumalawati Edwin Widyanto Daniwijaya Iva Fitriana Nida Budiwati Pramuko Citra Indriani Dwi Satria Wardana Warsito Tantowijoyo Riris Andono Ahmad Adi Utarini Eggi Arguni Copyright (c) 2023 Utari Saraswati, Endah Supriyati, Ayu Rahayu, Anwar Rovik, Irianti Kurniasari, Rio Hermantara, Dian Aruni Kumalawati, Edwin Widyanto Daniwijaya, Iva Fitriana, Nida Budiwati Pramuko, Citra Indriani, Dwi Satria Wardana, Warsito Tantowijoyo, Riris Andono Ahmad, Adi Utarini, Eggi Arguni https://creativecommons.org/licenses/by/4.0 2023-08-14 2023-08-14 20 2 117 117 10.5994/jei.20.2.117 Mating disruption technology: An innovative tool for managing yellow stem borer (Scirpophaga incertulas Walker) of rice in Indonesia https://jurnal.pei-pusat.org/index.php/jei/article/view/783 <p>Yellow stem borer (YSB) of rice, <em>Scirpophaga incertulas </em>Walker, causes significant damage to rice production in Indonesia. YSB management largely depends on insecticide applications and alternative management practices are very few and impractical. Pheromone-mediated mating disruption (MD) is a new way to manage YSB while reducing dependence on conventional insecticides. Pheron<sup>™</sup> RSB is an low-density polyethylene (LDPE) MD dispenser, containing 1.25 g of a four-way blended insect sex pheromone components - Z11-Hexadecenal, Z9-Hexadecenal, Z13-Octadecenal, Z9-Octadecenal in a ratio of 75, 8.6, 9.4, and 7 respectively. The objective is to evaluate MD by Pheron™ RSB as a tool for season-long management of YSB. Dispensers were applied at a rate of 20 dispensers/ha in a minimum area of 4 ha. We conducted these trials at 74 locations, non-replicated, across Java, in the wet and dry seasons of 2020–2021 where YSB are endemic and problematic. Efficacy of MD in pheromone foundational practice (PFP) was compared to conventional grower practice (CGP). Trap reduction, a measure of MD was significantly higher (&gt;70%) in PFP as compared to CGP. A major benefit of MD is reduction in damage. Significantly lower damage to rice tillers (40–46%) was seen in PFP compared to CGP. Lower damage in PFP likely protected yield by 0.43 to 0.76 ton/ha compared to CGP. Compared to PFP, CGP required 40–56% higher insecticide applications to manage YSB. Pheron<sup>™</sup> RSB provided season-long MD and proved to be a powerful tool for integrated management of YSB.</p> Muhamad Iqbal Maulana Marman Fridia Arintya Kristin Broms Thomas Clark Lakshmipathi Srigiriraju Copyright (c) 2023 Muhamad Iqbal, Maulana Marman, Fridia Arintya, Kristin Broms, Thomas Clark, Lakshmipathi Srigiriraju https://creativecommons.org/licenses/by/4.0 2023-08-14 2023-08-14 20 2 129 129 10.5994/jei.20.2.129. Variasi perilaku mencari makan pada semut rangrang Oecophylla smaragdina (Fabricius) pada habitat yang berbeda https://jurnal.pei-pusat.org/index.php/jei/article/view/760 <p>Weaver ants (<em>Oecophylla smaragdina </em>(Fabricus)) are carnivorous insects and play a role as biocontrol agents in urban habitats. This study aims to analyze the foraging behavior of <em>O. smaragdina </em>in two different habitats, namely urban and non-urban. The two locations are characterized by differences in the intensity of human interaction and the distance between the trees and the tree canopy. The behavioral observation method uses all sampling events with five behavioral categories: lurking (M1), approaching (M2), carrying (M3), communicating between ants in a colony (K1), and competition with other ants (K2). We tested three different types of feed: chick scraps, <em>Tenebrio molitor</em> Linnaeus larvae, and sugar. The results showed that there were three sequences of foraging behavior in urban areas, namely: (1) M1, M2, K1, M3, (2) M1, M2, K1, M3, K2, and (3) M1, M2, K2, M3. Meanwhile, there is only one pattern in non-urban areas, namely M1, M2, M3. Approaching food (M2) and lurking food (M1) were the dominant responses of <em>O. smaragdina</em> in urban and non-urban areas. In urban areas, broiler chickens were the type of feed most approached by ants, followed by sugar and beetle larvae. <em>O. smaragdina</em> in non-urban areas also preferred chicken pieces as feed compared to the others, but only one individual <em>O. smaragdina</em> approached (M2) chicken pieces and none approached the others. Foraging ants are more active at high temperature and low humidity, compared to low temperature and high humidity. Therefore, the foraging behavior of <em>O. smaragdina </em>is influenced by habitat factors.</p> Rezki Rezki Nur Aoliya Fadliansyah Fadliansyah Siti Latifa Wulandari David Reinhard Jesajas Rika Raffiudin Copyright (c) 2023 Rezki Rezki, Nur Aoliya, Fadliansyah Fadliansyah, Siti Latifa Wulandari, David Reinhard Jesajas, Rika Raffiudin https://creativecommons.org/licenses/by/4.0 2023-08-24 2023-08-24 20 2 141 141 10.5994/jei.20.2.141 Analisis filogenetik Hyposidra talaca nucleopolyhedrovirus (HytaNPV) yang diisolasi dari perkebunan teh Gunung Mas, Bogor, Jawa Barat dan virulensinya terhadap Hyposidra talaca Walker https://jurnal.pei-pusat.org/index.php/jei/article/view/779 <p><em>Hyposidra talaca</em> (Walker) is an important pest of tea plant. <em>H. talaca</em> can cause losses of between 40–100% in the dry season if proper control is not carried out. <em>H. talaca</em> has natural enemies such as predators, parasitoids, and pathogens. One of the entomopathogens is NPV. This study aims to obtain molecular characteristics through DNA polymerase sequences and determine the virulence level of NPV isolates from <em>H. talaca</em>. The same species from different locations can have genetic variability. Therefore, molecular characterization by polymerase chain reaction (PCR) on DNA polymerase sequences is one way to study the genetics of <em>Hyta</em>NPV. NPV was isolated from infected <em>H. talaca</em> larvae collected from the field. The DNA isolates were used as templates for PCR for DNA polymerase gene amplification with an amplicon target of ±1,000 bp. A sequencing process followed the PCR provides nucleotide sequence. <em>Hyta</em>NPV DNA polymerase sequencing results were aligned with GenBank’s BLAST data to provide information on the relationship of <em>Hyta</em>NPV to NPVs isolated from other regions. Based on molecular character analysis using DNA polymerase gene sequence, <em>Hyta</em>NPV Bogor has a homology level of 93.9% with <em>Hyta</em>NPV isolated from India. <em>Hyta</em>NPV Bogor has a genetic relationship with the NPV that infects <em>Buzura suppressaria</em> from China and Australia. <em>Hyta</em>NPV Bogor is similar to the NPV that infects <em>H. talaca </em>from India. The bioassay of <em>Hyta</em>NPV isolate against <em>H. talaca</em> showed the highest LT<sub>50</sub> value of 1.92 days was found in concentration of 1.58 x 107 POBs/ml in second instar larvae.</p> R. Yayi Munara Kusumah Fitrianingrum Kurniawati Eka Dana Kristanto Franciskus Parasian Michael Christian Copyright (c) 2023 R. Yayi Munara Kusumah, Fitrianingrum Kurniawati, Eka Dana Kristanto, Franciskus Parasian, Michael Christian https://creativecommons.org/licenses/by/4.0 2023-09-11 2023-09-11 20 2 151 151 10.5994/jei.20.2.151 Potensi reproduksi dan morfometri lalat tentara hitam, Hermetia illucens (Linnaeus) (Diptera: Stratiomyidae) yang dipelihara pada kotoran ayam dan kotoran domba https://jurnal.pei-pusat.org/index.php/jei/article/view/777 <p>Livestock manure such as chicken and sheep manures causes environmental problems such as odor pollution and pathogens found in livestock manure are harmful to animal and human health. The black soldier fly (BSF) have the ability to convert various types of organic waste effectively including livestock manure. Information on the reproductive potential and morphometry of BSF reared on livestock manure is still lack, as important data for optimizing the use of BSF to manage livestock manure. This study aims to determine the reproductive potential and morphometry of BSF reared on sheep and chicken manures. This experimental study used completely randomized design with three treatments, consist of chicken manure, sheep manure, and chicken feed as control. The development time, morphometry, mating frequency, and BSF fecundity were assessed. The sheep manure treatment had the highest development time among other treatments. Adult BSF morphometry reared on the chicken feed had the highest morphometric values compared to the chicken manure and the sheep manure. BSF reared on chicken feed had the highest number of mating pairs (44 pairs) compared to those treated with sheep manure (31 pairs) and chicken manure (20 pairs). The highest egg fecundity was found in BSF treated with chicken feed (3,535 eggs). These suggested that chicken manure and sheep manure are suitable as growth media for BSF, while its reproductive potential is lower compared to chicken feed.</p> Ucu Julita Ida Kinasih Dwinda Andini Copyright (c) 2023 Ucu Julita, Ida Kinasih , Dwinda Andini https://creativecommons.org/licenses/by/4.0 2023-09-14 2023-09-14 20 2 161 161 10.5994/jei.20.2.161 Rapid assessments of the rice brown planthopper (Nilaparvata lugens Stal) outbreak in Semarang District, Central Java: Effects of farmers’ low KAP https://jurnal.pei-pusat.org/index.php/jei/article/view/768 <p>Rapid assessments on the outbreak of rice brown planthoppers (BPH) (Nilaparvata lugens Stal) were conducted to investigate the status of BPH populations and the causative factors behind the outbreak. These assessments served as the basis for developing a proper action program. The assessments took place in Banyubiru Sub-District, Semarang District covering four villages (Kebondowo, Rowoboni, Tegaron, Kebumen) in December 2013. We analyzed BPH data, along with information about farmers’ practices in managing pests collected through interviews conducted in January 2018 in Indramayu. Additionally, we examined data on the number of BPH-infested areas in Java from 2010 to 2020 and the stock of insecticides in Java in 2021. Simple statistical analyses were carried out. The BPH population had spread throughout Banyubiru and was present in all sampled plots. The average number of BPH eggs ranged from 115.25 to 379.65 per rice hill, while the BPH nymphs and imagoes ranged from 3.42 to 11.87 per rice hill. The relatively low nymphs to imagoes ratio might be influenced by the high BPH predator populations, which ranged from three to six individuals per rice hill. Suspected causes of BPH resistance and resurgence included the application of banned and improper insecticides, as well as the repeated use of the same insecticide active ingredients for an extended period. It is recommended to discontinue the mass spraying of insecticides to prevent further plant damage. In 2022, the Pest Control Movement has suggested replacing chemical insecticides with biological or natural pesticides. Intensive extension programs are strongly needed.</p> Hermanu Triwidodo Bonjok Istiaji Nurul Farida Efriani Lilik Retnowati Niky Elfa Amanatillah Copyright (c) 2023 Hermanu Triwidodo, Bonjok Istiaji, Nurul Farida Efriani, Lilik Retnowati, Niky Elfa Amanatillah https://creativecommons.org/licenses/by/4.0 2023-10-16 2023-10-16 20 2 173 173 10.5994/jei.20.2.137