Jurnal Entomologi Indonesia 2024-04-30T16:43:09+00:00 Prof. Dr. Damayanti Buchori, MSc. Open Journal Systems <p><strong>Jurnal Entomologi Indonesia </strong>(<a href="">P-ISSN: 1829-7722</a>; <a href="">E-ISSN: 2089-0257</a>) is an Indonesian-based scientific journal, published by the <a href="">Entomological Society of Indonesia</a>. JEI is issued every March, July, and November of each year. It publishes original research article, reviews, and short communications covering research results in the field of tropical entomology. The JEI was founded in 2004 and is now in its 18th year. The journal is supported by the <a href="">Entomological Society of Indonesia</a> in collaboration with the <a href="">Department of Plant Protection</a>, Faculty of Agriculture, IPB University.</p> <p>JEI is an open-access, peer-reviewed journal that has been Accredited (2022-2026) base on the Ministerial Decree of the Minister of Research and Technology/Head of National Research and Innovation Agency of the Republic of Indonesia No. 105/E/KPT/2022</p> <p>The JEI has been registered in Crossref, DOAJ, <a href="" target="_blank" rel="noopener">SINTA Rank 2</a>, Google Scholar, Portal Garuda, CiteFactor, and other scientific databases. </p> The characterization of Apis cerana and Apis mellifera mrjp2 gene in Indonesia and the phylogeny relationship with mrjp family genes 2024-01-18T03:37:15+00:00 Nurul Insani Shullia Tia Vina Febiriani Rika Raffiudin Candra Widjaja Tri Atmowidi <p>The <em>major royal jelly protein 2</em> (<em>mrjp2</em>) gene is one of the molecular markers that can discriminate between <em>Apis cerana</em> Fabricius and <em>A. mellifera </em>Linnaeus. Due to the lack of <em>mrjp</em>2 gene sequences registered in GenBank for Indonesian <em>A. cerana</em> and <em>A. mellifera</em>, DNA characterization and bioinformatics analysis were needed. This research aimed to characterize the exon-intron organization of <em>mrjp2</em> genes for both Indonesian bee species and analyze the phylogenetic relationship with other <em>mrjp</em> family genes. DNA samples of <em>Apis cerana</em> and <em>A. mellifera,</em> collected from Bogor, were amplified using MF-MR primer at annealing temperatures of 47 <sup>o</sup>C and 50 <sup>o</sup>C, respectively. The length of <em>A. cerana</em> and <em>A. mellifera</em> DNA sequences were 579 and 597 bp, respectively. The DNA sequences of both species were comprised of partial exons 1, introns 1, exons 2, introns 2, and partial exon 3. The number of putative amino acids of <em>A. cerana</em> and <em>A. mellifera</em> <em>mrjp</em>2 genes were 111 and 123, respectively. We confirmed that the partial MRJP2 putative amino acids of both honey bees belong to the MRJP family and contained the peptide signal in the 14 first amino acid sites. Nucleotide variation of <em>the mrjp2</em> gene in <em>A. cerana</em> was higher than <em>A. mellifera</em>. Phylogenetic tree construction showed that <em>A. cerana</em> and <em>A. mellifera </em>form a monophyletic clade with the <em>A. mellifera</em> <em>mrjp7</em> gene and another <em>mrjp</em> family gene clustered as reported in a previous study.</p> 2024-04-30T00:00:00+00:00 Copyright (c) 2024 Nurul Insani Shullia, Tia Vina Febiriani, Rika Raffiudin, Candra Widjaja, Tri Atmowidi Pengaruh pemberian sampah organik terhadap pertumbuhan dan kandungan astaxanthin pada pupa black soldier fly (Hermetia illucens (Linnaeus)) 2024-01-03T06:11:33+00:00 Arkhey Sidauruk Aniek Prasetyaningsih Kukuh Madyaningrana <p>Black soldier flies (<em>Hermetia illucens</em> (Linnaeus)) or BSF are insects that process organic waste, with larvae rich in nutrients and potential as an alternative protein source for food and feed. Astaxanthin, an antioxidant found in plants, algae, and crustaceans, is also assumed to be present in insects like BSF. This study examined the impact of feeding different organic wastes on BSF larval growth and astaxanthin content in its pupae. The experimental design involved four treatments: fruit and vegetable scraps (BS), tofu pulp (AT), rice leftovers (N), and a mix of fruit and vegetable scraps, tofu pulp and rice left-over (C), with animal feed T51 as control. Each treatment was repeated three times. Growth parameters of BSF such as weight, length, and larval characteristics, along with the chemical content of pupae, including astaxanthin, were measured. Pupae were extracted using a triphasic maceration method with n-heptane, ethyl acetate, acetonitrile, butanol-l, and distilled water. Compound identification was done using terpenoid qualitative tests, thin layer chromatography (TLC), and liquid chromatography-mass spectrometry (LC-MS). The findings revealed that fruit and vegetable scraps (BS) significantly increased larval body size, with a weight of 0.09 ± 0.04 grams/larvae and a length of 1.49 ± 0.31 cm/larvae. LC-MS analysis detected lutein derivatives, anisodamine, resveratrol derivatives, dehydroarginine, and astaxanthin derivatives with a molecular mass of 597.23 m/z in BSF larvae fed on fruit and vegetable scraps.</p> 2024-06-04T00:00:00+00:00 Copyright (c) 2024 Arkey Sidauruk, Aniek Prasetyaningsih, Kukuh Madyaningrana